Enzyme Engineering

Enzymes are fascinating biocatalysts, which impress by their extraordinary chemo-, regio- and stereoselectivity. In eons nature formed an enormous diversity of these perfectly fitting instruments. In our enzyme technology research we attempt to explore a modest part of this variety as tailored tools for organic synthesis. Although our research encompasses the natural selectivity of enzymes, sometimes they have to be optimized by mutagenesis with respect to our synthetical endeavors. Our focus lies on the use of hydrolases, lyases as well as oxidoreductases.

Selected Publications

Marc Hayes, Kevin Bochinsky, Lisa Seibt, Lothar Elling, Jörg Pietruszka, J. Biotechnol., 2017, DOI: dx.doi.org/10.1016/j.jbiotec.2017.02.005, "Development of a colorimetric assay for glycosynthases"

Carolin Bisterfeld, Thomas Classen, Irene Küberl née Kullartz, Birgit Henßen, Alexander Metz, Holger Gohlke, Jörg Pietruszka, PLoS ONE, 2016, 11(6), e0156525, "Redesigning aldolase stereoselectivity by homologous grafting"

Markus Dick, Rudolf Hartmann, Oliver H. Weiergraeber, Carolin Bisterfeld, Thomas Classen, Melanie Schwarten, Philipp Neudecker, Dieter Willbold, Jörg Pietruszka, Chem. Sci. 2016, 7, 4492-4502; "Mechanism-based Inhibition of an Aldolase at High Concentrations of its Natural Substrate Acetaldehyde: Structural Insights and Protective Strategies"

Markus Dick, Oliver H. Weiergräber, Thomas Classen, Carolin Bisterfeld, Julia Bramski, Holger Gohlke, Jörg Pietruszka, Sci. Rep. 2016, 6, 17908 "Trading off stability against activity in extremophilic aldolases"

Elisabeth Rüthlein, Thomas Classen, Lina Dobnikar, Melanie Schölzel, Jörg Pietruszka, Adv. Synth. Catal. 2015, 357, 1775-1786; "Finding the Selectivity Switch - A Rational Approach towards Stereocomplementary Variants of the Ene Reductase YqjM"

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